MDA5与TLR3信号转导在IBDV致雏鸡法氏囊免疫损伤中的作用机制

项目名称： MDA5与TLR3信号转导在IBDV致雏鸡法氏囊免疫损伤中的作用机制

项目编号： No.31272533

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 张瑞莉

作者单位： 东北农业大学

项目金额： 80万元

中文摘要： IBDV为dsRNA病毒，可造成感染雏鸡法氏囊严重损伤而导致雏鸡发生免疫抑制。本研究从能够特异性识别dsRNA病毒的模式识别受体（MDA5与TLR3）及其信号转导入手，以IBDV感染雏鸡法氏囊损伤为切入点，采用实时荧光定量PCR、免疫电镜、免疫酶及传统病理学方法，检测IBDV感染雏鸡、IBDV中等毒力活疫苗免疫对照雏鸡和空白对照雏鸡法氏囊MDA5与TLR3的定位与表达变化；MDA5与TLR3信号转导通路中衔接蛋白分子（IPS1、MyD88、TRIF）、转录因子（NF-κB、IRF3）及其诱导产物（TNF-α、IL-1、IL-6、IFN）表达变化；体液和细胞免疫以及组织病理变化，明确IBDV感染雏鸡法氏囊免疫损伤与MDA5、TLR3信号转导通路活化的内在联系，进一步从雏鸡体内抗病毒模式识别及信号转导的角度在分子水平阐明IBDV致雏鸡免疫抑制机制，为防制雏鸡IBD提供新的科学思路。

中文关键词： MDA5与TLR3信号转导；IBDV；雏鸡；法氏囊；免疫抑制

英文摘要： The severe bursal lesions is the key point of the immunosuppression in chikens infected with infectious bursal diaease virus (IBDV), which belongs to double-stranded ribonucleic acid (dsRNA) virus. Melanoma differentiation associated gene 5 (MDA5) and toll-like receptor 3 (TLR3) are the pattern recognition receptor detecting for dsRNA virus. In the context, MDA5 and TLR3 of bursas are considered to play important roles in the immunosuppression mechanism in IBDV-infected chickens and need to be certified experimentally. Two-week-old specific pathogen free chickens were inoculated intraocularly and perorally with standard IBDV strain and live vaccine IBDV, respectively. The dynamic cellular and subcellular localization of MDA5 and TLR3, expression of interrelated signal molecules (including IPS1, MyD88, TRIF, NF-κB and IRF3) and their induced products (including TNF-α, IL-1, IL-6 and IFN ) were detected by the methods of real-time fluorescence quantitative PCR, immune electron microscopy techniques. At the same time, the humoral immunity, cellular immunity and tissue morphologic changes of bursas will be the examinational contents in the chickens with immunoenzyme and traditional pathological techniques. The inherent connections of immunolession and signal transduction of MDA5 and TLR3 in bursas of IBDV-infected c

英文关键词： signal transduction of MDA5 and TLR3；infectious bursa diseaes virus；chicken；bursa；immunosuppression