基于高荧光性能碳点的免疫荧光探针构建及其在微生物检测中的应用研究

项目名称： 基于高荧光性能碳点的免疫荧光探针构建及其在微生物检测中的应用研究

项目编号： No.51303210

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 一般工业技术

项目作者： 刘长军

作者单位： 中国人民解放军军事医学科学院

项目金额： 25万元

中文摘要： 研制低毒、强荧光、高稳定性的荧光标记物对于推动荧光免疫技术的发展至关重要。碳点（C-dots）是一种环境友好、生物安全的新型光致发光碳纳米材料，在能量转换、生物医学和高灵敏度检测等领域具有独特的优势。本项目拟在前期研究基础上，采用微波辅助法制备水溶性的氨基化碳点（Amino-CD）与羧基化性碳点(Carboxyl-CD)，系统分析微波功率、微波时间、反应物配比等工艺条件对碳点结构、组成、尺寸的影响规律，揭示碳点生长模型；探索碳点结构、组成、尺寸、表面状态以及金属掺杂物类型与碳点荧光性能之间的内在联系，阐明碳点荧光机理；以大肠杆菌为微生物模型，通过EDC/NHS 化学偶联法将大肠杆菌抗体修饰到碳点表面，构建免疫荧光探针，用于大肠杆菌的特异性识别与检测，为研制具有自主知识产权的新型、高效、低毒、环保荧光标记物奠定基础。

中文关键词： 碳点；荧光探针；抗体；免疫荧光分析；

英文摘要： Fluoroimmunoassay is a popular method for the detection of bacteria, virus and parasite attributed to its high selectivity and sensitivity. However, the use of this method is greatly limited by the fluorescent performance of the labeling dyes. Therefore, construction of low toxic, strong and stable fluorescent labels is critical to the development of fluoroimmunoassay technique. Recently, carbon dot (C-dot), an emerging fluorescent carbon nanomaterial, has drawn tremendous attention owing to its advantages in stable photoluminescence (PL), broad excitation spectra, tunable emission spectra, low cytotoxicity compared with traditional organic dyes. Moreover, its environmental friendly characteristics signify that C-dots are advantageous over toxic metal-based semiconductor quantum dots (QDs) in actual applications. Therefore C-dot is a promising alternative to QDs in energy conversion/storage, bioimaging, drug delivery, sensors and diagnostics. To date, however, there have been few reports on quantitative detction for microorganism using fluorescent probe based on C-dots. In this project, we propose to explore a simple, green and economical route to build C-dots with strong fluorescence and controllable versatile surface groups and will investigate its potential application in immundetection for the first time. We

英文关键词： carbon dots；fluorescent probe；antibody；fluoroimmunoassay；