面向食品安全的霉菌毒素蓝光光盘检测技术研究

项目名称： 面向食品安全的霉菌毒素蓝光光盘检测技术研究

项目编号： No.21505098

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 高分子科学

项目作者： 李晓春

作者单位： 太原理工大学

项目金额： 21万元

中文摘要： 食品安全是关系到国计民生的大事，全世界各国在食品安全技术方面投入了极大的人力与物力，其中准确、快速的现场检测技术是保证食品安全最为关键的手段之一。在众多食品安全风险因素中，以霉菌毒素的污染及危害最为严重。标准色谱法是目前最为准确的检测方法，但所需设备昂贵、操作复杂、耗时长、需专业人员操作，难以实现现场检测。. 蓝光光盘（Blu-Ray Disc）是继DVD之后的新一代光盘格式之一，用以存储高质量的影音以及高容量的数据。在利用各类光盘（CD/DVD）进行数据读写时，人们创造性地将光盘作为生物分子结合载体，并利用计算机光驱精密的读取与伺服系统进行生物分子的检测。在我们坚实的前期研究基础上，本申请项目提出了基于蓝光光盘（Blu-Ray Disc）技术的霉菌毒素微阵列检测方法，并且利用普通打印机实现霉菌毒素检测微阵列的自动化制备。这种方法具有成本低，操作简单，易推广，在线快速检测的优点。

中文关键词： 食品安全；蓝光光盘；霉菌毒素；快速检测

英文摘要： Food safety is critical to people’s life, for which troumendous amount of efforts has been devoted to develop monitoring or diagnostic techniques. An accurate, rapid screening method is the must to ensure today’s food safety. Within many food safety concerns, the mycotoxin contamination is one of the most serious issues. Conventional chromatography is the most accurate detection and quantitation method, but it is typically time-consuming, labor-intensive, and limited to well-equipped laboratories as it requires special instrumentation and highly trained personnel..Optical disc is the most successful data storage technology in the 20th century; Blu-Ray is the newest format with the highest storage capacity and the best reading accuracy. We have in the past explored application of optical discs for molecular screening: the discs offer versatile substrate materials, and optical drives can be adapted as quantitative optical reading devices for microarray biochips. .Based on our previous successful studies, we propose herein the development of a bioassay platform based entirely on standard blu-ray disc (BD) technology for quantitative mycotoxin detection and quantitation. We envision to realize the fabrication of the assay disc using a standard inkjet printer and the signal readout with conventional blu-ray drive. The proposed technology promises to be a versatile analytical/diagnostic tool for medical screening, on-site food/water safety testing, and remote environmental monitoring.

英文关键词： Food Safety;Blu-ray Disc;Mycotoxins;Rapid test