高铝暴露AA TCR谱系和信号传导通路改变特点及其与预后相关性研究

项目名称： 高铝暴露AA TCR谱系和信号传导通路改变特点及其与预后相关性研究

项目编号： No.81460026

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 陆翔

作者单位： 右江民族医学院

项目金额： 50万元

中文摘要： 我市为中国铝工业基地，再生障碍性贫血(aplastic anemia,AA)在我市发病逐年增高。高铝或长期铝暴露对人体免疫功能可产生不利影响。TCR-CD3复合物在T细胞的激活、增殖及功能改变中起着关键作用。AA是免疫异常介导的自身免疫病，T细胞的寡克隆性增殖是其特点。我们在前期研究提示AA CD3表达的异常可能与T细胞免疫功能障碍有关，高铝暴露AA的临床特征和免疫学改变有别于原发性AA的基础上，利用RT-PCR-基因扫描测定高铝暴露AA CD4+T、CD8+T细胞各TCR谱系、CD3复合物基因的表达及克隆性增殖T细胞性质，分析高铝暴露地区AA患者TCR谱系及信号传导通路相关分子CD3表达及T细胞克隆分布特点，进一步系统了解高铝暴露AA更为全面的分子和细胞免疫学指标，深入探讨高铝相关的免疫学改变及其对AA的影响。为高铝暴露或血铝增高的AA患者临床和T细胞免疫功能异常改变提供基础研究资料。

中文关键词： 铝；T细胞谱系；T细胞受体；CD3基因；再生障碍性贫血

英文摘要： Our city is located in aluminium industry base in China,the morbidity of (aplastic anemia, AA) have increased in recent years in our city.High aluminum or long-term exposure can produce adverse effect to the body's immune function.AA is an autoimmunity disease mediated by T cell immune abnormalities , the cloned proliferation of T cells affecting the bone marrow hematopoietic is one of its characteristics, the TCR-CD3 compound plays a important role in T cell's activation, proliferation and function change .In our early study we found that among the patients with primary AA ,the TCR spectrum and the change of TCR signaling pathways may be associated with T cell immune function . The clinical features of AA in high aluminium exposed area and T cell subgroup changing is different from the primary AA, on which basis this study will use RT-PCR genescan to measure the spectrum of CD4 + T and CD8 + T cells , the character of the cloned proliferation of T cells and the gene expression of CD3 complex .Analysising the differences of cellular immunological indexes about the spectrum and the signal pathway of TCR between the patients with AA under the condition of high aluminium exposure and the ones not. To discuss the immunological changes and the impact on AA related to the high alumina exposed.Providind the basis for the abnormal changes of immunologic function in patients with AA under the condition of high aluminium exposure.

英文关键词： Aluminum;T cell repertoire;T cell receptor;CD3 gene;Aplastic anemia