基于拓扑结构的魔芋葡甘聚糖链稳定性研究

项目名称： 基于拓扑结构的魔芋葡甘聚糖链稳定性研究

项目编号： No.31471704

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 庞杰

作者单位： 福建农林大学

项目金额： 90万元

中文摘要： 多糖分子链动态变化直接影响着食品品质，但由于糖链结构复杂且不规则，至今难以实现对其动态变化过程的调控；基于前一基金研究发现魔芋葡甘聚糖（KGM）与离子形成具热稳定性的拓扑缠结，本项目提出链耦合穿孔假说，即KGM经耦合穿孔形成具有热稳定性的拓扑缠结骨架。围绕KGM分子链→链耦合穿孔→建立链稳定性参数的数学模型→多尺度模拟验证的思路，拟通过研究KGM链耦合穿孔形成的动态过程，构建拓扑缠结骨架，形成稳定性链结构。采用荧光探针与物性学方法判断是否形成穿孔，对KGM拓扑缠结骨架进行多尺度的计算机模拟，量化KGM动态拓扑缠结骨架的具体参数，构建构象的数学稳定性模型。项目结合多尺度模拟验证与流变学、波谱学等方法探索链耦合穿孔与拓扑缠结的关系，从而探究链稳定性机理。本项目研究有望揭示KGM分子链稳定性机理，为后续在加工过程中对KGM的动态调控以提高食品品质奠定理论基础。

中文关键词： 魔芋葡甘聚糖；拓扑结构；链耦合穿孔；多尺度模拟；稳定性操控

英文摘要： The dynamic changes of the polysaccharide chain directly affect food quality characteristics in the processing. It is very important to provide some theoretical basis. Polysaccharide chain has a very complex intra-molecular structure and it is not easy to characterize the complex as well as to control its dynamic. Current studies have been focus on their separation, structural identification and functionality, but rarely in the field of regulation of its dynamic stability. Based on a former project, which reveals the thermostatbility and topology of Konjac Glucomannan (KGM), we proposed a hypothesis of the chain coupling hole in KGM's topology structure. This study will be conducted with the idea of KGM molecular chain -- the 'coupling perforation' of the topology chain -- multi-scale evolution and simulation - regulation of dynamic stability by using the fluorescence probe and field verification to determine the perforation. This study is designed to reveal idea and methodology to analysis coupled perforation (node) of molecular chain by mathematic model. On the other hand, this project will explore the dynamic relationships between conformation and properties of polysaccharide chain in order to realize the selection and manipulation of the molecules. In summary, this study has significance for providing the theoretical basis of stability and performance as well as for future research in dynamic molecular control process.

英文关键词： Konjac glucomannan;topology;multi-scale simulation;modeling of chain conformation;stability control.