模块化设计多功能蛋白纳米线及超灵敏生物传感应用

项目名称： 模块化设计多功能蛋白纳米线及超灵敏生物传感应用

项目编号： No.31200755

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 生物物理、生化与生物分子学、生物力学与组织工程

项目作者： 门冬

作者单位： 中国科学院武汉病毒研究所

项目金额： 23万元

中文摘要： 对病原分子进行高灵敏度检测一直是生物传感的重要需求之一。近年来，纳米材料在生物传感中的广泛应用，显著提高了病原检测的灵敏度。但无机纳米材料通常功能单一，需要功能生物分子修饰才能用于生物传感。本申请拟利用淀粉样蛋白自组装的性质和蛋白质工程技术，模块化设计可便捷更换功能配体的多功能蛋白纳米线。新的纳米线由自组装元件和功能元件构成。功能元件通过接头装置直接展示在自组装元件形成的通用纳米线底盘模块上成为功能模块。通过体外操纵自组装，可将特异性识别模块和信号放大模块整合在纳米线底盘上，得到同时具有特异结合能力和信号放大能力的多功能蛋白纳米线。将不同功能模块组合，多功能纳米线可适用于多种形式和不同目标分子的生物传感，成为普遍适用的系列化高灵敏蛋白纳米线传感器件。该方法利用模块化设计和分子自组装有效整合多种功能配体，使纳米线具有更多的功能选择以完成不同的任务，也为发展多功能纳米器件提供了新的技术路线。

中文关键词： 自组装；蛋白纳米线；铁蛋白纳米颗粒；多功能蛋白纳米结构；高灵敏免疫分析

英文摘要： There is substantial interest in the use of biosensing to detect pathogen at ultralow levels. Recently, nanomaterials have been widely used in biosensing and have substantially improved sensitivity. However, most of these materials require functional biomolecular modification before they can be applied in biosensors. A new modularity multipurpose protein nanowire is designed in this application which is built by engineered self-assembly amyloid protein. The protein nanowire is composed by self-assembly components and functional elements that can be bound to each other through fused adapter peptide. The functional module is formed by functional elements binding to the surface of protein nanowire chassis which consist of self-assembly components. By controlling the self-assembly process, the resultant protein nanowire possesses a specific recognition module and a signal amplification module, allowing a dramatic increase of the signal when recognition module bind to target. Protein nanowire for different tasks can be easily built by combinating of different features. The modular designed multipurpose protein nanowire could be a series of universally applicable high-sensitivity biosensing systems. This method effectively integrates the different functions through modularity self-assembly. The new protein nanowire ha

英文关键词： Self-assembly；protein nanowire；ferritin nanoparticle；multi-functional protein nanostructure；highly-sensitive immunoassay