We propose a method to reconstruct the 3-D molecular structure from micrographs collected at just one sample tilt angle in the random conical tilt scheme in cryo-electron microscopy. Our method uses autocorrelation analysis on the micrographs to estimate features of the molecule which are invariant under certain nuisance parameters such as the positions of molecular projections in the micrographs. This enables us to reconstruct the molecular structure directly from micrographs, completely circumventing the need for particle picking. We demonstrate reconstructions with simulated data and investigate the effect of the missing-cone region. These results show promise to reduce the size limit for single particle reconstruction in cryo-electron microscopy.
翻译:我们建议一种方法,用在冷冻-电子显微镜中随机锥形倾斜方案中仅从一个样本倾斜角度收集的显微镜来重建三维分子结构。我们的方法是用对显微镜的自动关系分析来估计分子的特性,这些微镜在某些干扰参数下是无差异的,例如微微镜中的分子预测位置。这使我们能够直接从显微镜中重建分子结构,完全避免摘取粒子的需要。我们用模拟数据来进行重新组合,并调查缺失孔区域的效果。这些结果表明,有可能降低在显微镜中进行单粒重建的规模限制。